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32. Photoproximity labeling of endogenous receptors in the live mouse brain in minutes
Mikiko Takato, Seiji Sakamoto, Hiroshi Nonaka, Fatima Yuri Tanimura Valor, Tomonori Tamura*, Itaru Hamachi*
Nature Chemical Biology, just accepted
31. Protein-labeling reagents selectively activated by copper(I)
Rong Cheng, Yuki Nishikawa, Takumi Wagatsuma, Taiho Kambe, Yu-ki Tanaka, Yasumitsu Ogra, Tomonori Tamura*, Itaru Hamachi*
ACS Chemical Biology, 19, 6, 1222-1228 (2024) doi: 10.1021/acschembio.4c00011
30. Bioorthogonal chemical labeling of endogenous neurotransmitter receptors in living mouse brains
H. Nonaka, S. Sakamoto, K. Shiraiwa, M. Ishikawa, T. Tamura, K. Okuno, T. Kondo, S. Kiyonaka, E. A. Susaki, C. Shimizu, H. R. Ueda, W. Kakegawa, I. Arai, M. Yuzaki, I. Hamachi*
Proceeding of National Academy of Science of USA, 6: 2313887121 (2024) doi: 10.1073/pnas.2313887121
29. Lysine-reactive N-acyl-N-aryl sulfonamide warheads: Improved reaction properties and application in the covalent inhibition of an ibrutinib-resistant BTK mutant
Masaharu Kawano, Syunsuke Murakawa, Kenji Higashiguchi, Kenji Matsuda, Tomonori Tamura*, Itaru Hamachi*
Journal of the American Chemical Society, 145, 48, 26202–26212 (2023) doi: 10.1021/jacs.3c08740
28. Endogenous cell-surface receptor modification by metal chelation-assisted pyridinium oxime catalyst
Vikram Thimaradka, Hayata Utsunomiya, Tomonori Tamura*, Itaru Hamachi*
Organic Letters, 25, 12, 2118-2122 (2023) doi:10.1021/acs.orglett.3c00541
27. Organelle-selective click labeling coupled with flow cytometry allows pooled CRISPR screening of genes involved in phosphatidylcholine metabolism
Masaki Tsuchiya, Nobuhiko Tachibana, Kohjiro Nagao, Tomonori Tamura*, Itaru Hamachi*
Cell Metabolism , 35, 6, 1072-1083 (2023) doi:10.1016/j.cmet.2023.02.014
26. Revisiting PFA-mediated tissue fixation chemistry: FixEL enables trapping of small molecules in the brain to visualize their distribution changes
Hiroshi Nonaka, Takeharu Mino, Seiji Sakamoto, Jae Hoon Oh, Yu Watanabe, Mamoru Ishikawa, Akihiro Tsushima, Kazuma Amaike, Shigeki Kiyonaka, Tomonori Tamura, A. Radu Aricescu, Wataru Kakegawa, Eriko Miura, Michisuke Yuzaki, Itaru Hamachi*
Chem , 9, 2, 523-540 (2023) doi:10.1016/j.chempr.2022.11.005
25. Light-induced stabilization of microtubules by photo-crosslinking of Tau-derived peptide
Soei Watari, Hiroshi Inaba, Tomonori Tamura, Arif Md. Rashedul Kabir, Akira Kakugo, Kazuki Sada, Itaru Hamachi, Kazunori Matsuura
Chemical Communications , 58, 9190 - 9193 (2022) doi:10.1039/D2CC01890J
24. Enhanced Suppression of a Protein–Protein Interaction in Cells Using Small-Molecule Covalent Inhibitors Based on an N-Acyl-N-alkyl Sulfonamide Warhead
Tsuyoshi Ueda, Tomonori Tamura, Masaharu Kawano, Keiya Shiono, Fruzsina Hobor, Andrew J. Wilson, and Itaru Hamachi*
Journal of the American Chemical Society , 143 (2021) doi:10.1021/jacs.1c00703
23. Site-specific covalent labeling of His-tag fused proteins with N-acyl-N-alkyl sulfonamide reagent
Vikram Thimaradka, Jae Hoon Oh, Christina Heroven, A. Radu Aricescu, Michisuke Yuzaki, Tomonori Tamura*, Itaru Hamachi*
Bioorganic & Medicinal Chemistry , 30, 115947 (2021) doi:10.1016/j.bmc.2020.115947
22. Organelle membrane-specific chemical labeling and dynamic imaging in living cells
Tomonori Tamura, Alma Fujisawa, Masaki Tsuchiya, Yuying Shen, Kohjiro Nagao, Shin Kawano, Yasushi Tamura, Toshiya Endo, Masato Umeda & Itaru Hamachi*
Nature Chemical Biology ,16, 12, 1361-1367 (2020) doi: 10.1038/s41589-020-00651-z
21. Imaging and profiling of proteins under oxidative conditions in cells and tissues by hydrogen-peroxide-responsive labeling
Hao Zhu, Tomonori Tamura, Alma Fujisawa, Yuki Nishikawa, Rong Cheng, Mikiko Takato, and Itaru Hamachi*
Journal of the American Chemical Society , 142, 37, 15711–15721(2020) doi:10.1021/jacs.0c02547
20. Development of a Photoactivatable Proximity Labeling Method for the Identification of Nuclear Proteins
Tomonori Tamura*, Mikiko Takato, Keiya Shiono, Itaru Hamachi*
Chemistry Letters , 49, 145-148 (2020) doi:10.1246/cl.190804
19. Development of a cell-based ligand-screening system for identifying Hsp90 inhibitors
Tsuyoshi Ueda, Tomonori Tamura*, Itaru Hamachi*
Biochemistry , 59, 2, 179-182 (2020) doi:10.1021/acs.biochem.9b00781
18. Development of a nitric oxide-responsive labeling reagent for proteome analysis of live cells
Yuki Nishikawa, Takayuki Miki, Masashi Awa, Keiko Kuwata, Tomonori Tamura, Itaru Hamachi
ACS Chemical Biology , 14, 3, 397–404 (2019) doi: 10.1021/acschembio.8b01021
17. Selective and reversible modification of kinase cysteines with chlorofluoroacetamides.
Naoya Shindo, Hirokazu Fuchida, Mami Sato, Kosuke Watari, Tomohiro Shibata, Keiko Kuwata, Chizuru Miura, Kei Okamoto, Yuji Hatsuyama, Keisuke Tokunaga, Seiichi Sakamoto, Satoshi Morimoto, Yoshito Abe, Mitsunori Shiroishi, Jose M. M. Caaveiro, Tadashi Ueda, Tomonori Tamura, Naoya Matsunaga, Takaharu Nakao, Satoru Koyanagi, Shigehiro Ohdo, Yasuchika Yamaguchi, Itaru Hamachi, Mayumi Ono and Akio Ojida
Nature Chemical Biology (2019) doi: 10.1038/s41589-018-0204-3
16. Chemistry for Covalent Modification of Endogenous/Native Proteins: From Test Tubes to Complex Biological Systems.
Tomonori Tamura, Itaru Hamachi
Journal of the American Chemical Society, Just accepted doi: 10.1021/jacs.8b11747
15. Chemical profiling of the endoplasmic reticulum proteome using designer labeling reagents.
Alma Fujisawa, Tomonori Tamura, Yuki Yasueda, Keiko Kuwata, Itaru Hamachi
Journal of the American Chemical Society, 140, 49, 17060-17070(2018)
14. Rapid labelling and covalent inhibition of intracellular native proteins using ligand-directed N-acyl-N-alkyl sulfonamide.
Tomonori Tamura, Tsuyoshi Ueda, Taiki Goto, Taku Tsukidate, Yonatan Shapira, Yuki Nishikawa, Alma Fujisawa, Itaru Hamachi
Nature Communications, 9, 1870 (2018)
13. Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding live cells.
Kazuma Amaike, Tomonori Tamura, Itaru Hamachi
Chem. Commun. Feature Article, 53, 11972-11983 (2017)
12. Affinity-guided oxime chemistry for selective protein acylation in live tissue systems.
Tomonori Tamura, Zhining Song, Kazuma Amaike, Shin Lee, Sifei Yin, Shigeki Kiyonaka, Itaru Hamachi
J. Am. Chem. Soc., 139, 14181-14191 (2017)
11. A set of organelle-localizable reactive molecules for mitochondrial chemical proteomics in living cells and brain tissues.
Yuki Yasueda, Tomonori Tamura, Alma Fujisawa, Keiko Kuwata, Shinya Tsukiji, Shigeki Kiyonaka, Itaru Hamachi
J. Am. Chem. Soc., 138, 7592-7602 (2016)
10. Nucleus-selective Chemical Proteomics Using Hoechst-tagged Reactive Molecules.
Yuki Yasueda, Tomonori Tamura, Itaru Hamachi
Chem. Lett., 45, 265-267 (2016)
9. Biomembrane-embedded catalysts for membrane-associated protein labeling on red blood cells.
Yuki Yasueda, Tomonori Tamura, Keiko Kuwata, Yousuke Takaoka, Itaru Hamachi
Chem. Lett., 44, 1673-1675 (2015)
8. Analysis of cell-surface receptor dynamics through covalent labeling by catalyst-tethered antibody.
Takahiro Hayashi, Yuki Yasueda, Tomonori Tamura, Yousuke Takaoka, Itaru Hamachi
J. Am. Chem. Soc., 137, 3360-3365 (2015)
7. Extended affinity-guided DMAP chemistry with finely-tuned acyl donor for intracellular FKBP12 labeling.
Zhining Song, Yousuke Takaoka, Yoshiyuki Kioi, Kazuhiro Komatsu, Tomonori Tamura, Takayuki Miki, Itaru Hamachi
Chem. Lett., 44, 333-335 (2015)
6. Live cell off-target identification of lapatinib using ligand-directed tosyl chemistry.
Kei Yamaura, Keiko Kuwata, Tomonori Tamura, Yoshiyuki Kioi, Yousuke Takaoka, Shigeki Kiyonaka, Itaru Hamachi
Chem. Commun., 50, 14097-14100
(2014)
5. Semisynthetic lectin−4-dimethylaminopyridine conjugates for labeling and profiling glycoproteins on live cell surfaces.
Takahiro Hayashi, Yedi Sun, Tomonori Tamura, Keiko Kuwata, Zhining Song, Yousuke Takaoka, Itaru Hamachi
J. Am. Chem. Soc., 135, 12252-12258 (2013)
4. Fluorophore labeling of native FKBP12 by ligand-directed tosyl chemistry allows detection of its molecular interactions in vitro and in living cells.
Tomonori Tamura, Yoshiyuki Kioi, Takayuki Miki, Shinya Tsukiji, Itaru Hamachi
J. Am. Chem. Soc., 135, 6782-6785 (2013)
3. Native FKBP12 engineering by ligand-directed tosyl chemistry: labeling properties and application to photo-cross-linking of protein complexes in vitro and in living cells.
Tomonori Tamura, Shinya Tsukiji, Itaru Hamachi
J. Am. Chem. Soc., 134, 2216-2226 (2012)
2. Quenched ligand-directed tosylate reagents for one-Step construction of turn-on fluorescent biosensors.
Shinya Tsukiji, Hangxiang Wang, Masayoshi Miyagawa, Tomonori Tamura, Yousuke Takaoka, Itaru Hamachi
J. Am. Chem. Soc., 131, 9046-9054 (2009)
1. Ligand-directed tosyl chemistry for protein labeling in vivo.
Shinya Tsukiji, Masayoshi Miyagawa, Yousuke Takaoka, Tomonori Tamura, Itaru Hamachi
Nat. Chem. Biol., 5, 341-343 (2009)
15. 金属イオン応答性タンパク質修飾試薬を用いたコンディショナルプロテオミクス
田村 朋則
ファルマシア, 59, 3, 190-195 (2023) doi: 10.14894/faruawpsj.59.3_190
14. システイン以外のアミノ酸残基を標的とする不可逆阻害薬
田村 朋則
日本薬理学雑誌, 157, 5, 383 (2022)
13. Chemical biology tools for imaging-based analysis of organelle membranes and lipids
Tomonori Tamura, Itaru Hamachi
Current Opinion in Chemical Biology, 70: 102182 (2022) doi: 10.1016/j.cbpa.2022.102182
12. Organelle-selective labeling of choline-containing phospholipids(CPLs) and real-time imaging in living cells
Masaki Tsuchiya, Tomonori Tamura, Itaru Hamachi*
Current Protocols, e105, Volume 1 (2021) doi: 10.1002/cpz1.105
11. Chemical Tools for Endogenous Protein Labeling and Profiling
Kazuki Shiraiwa, Rong Cheng, Hiroshi Nonaka, Tomonori Tamura, and Itaru Hamachi*
Cell Chemical Biology, 27, 8, 970-985(2020) doi:10.1016/j.chembio|.2020.06016
10. Chemical Proteomics for Subcellular Proteome Analysis
Hao Zhu, Tomonori Tamura, Itaru Hamachi
Current opinion in chemical biology, 48, 1-7 (2019)
9. Molecular Technologies in Life Innovation:Novel Molecular Technologies for Labeling and Functional Control of Proteins Under Live Cell Conditions.
Itaru Hamachi, Shigeki Kiyonaka, Tomonori Tamura, Ryou Kubota
Molecular Technology vol.2, 297-328 (2018)
8. Chemical Proteomics for Subcellular Proteome Analysis.
Hao Zhu, Tomonori Tamura, Itaru Hamachi
Current opinion in chemical biology, 48, 1-7 (2019)
7. In situ Construction of Protein-based Semisynthetic Biosensors.
Tsuyoshi Ueda, Tomonori Tamura, Itaru Hamachi
ACS Sensors, 3, 527-539 (2018)
6. リガンド指向性NASA化学による高速蛋白質ラベリングとコバレントドラッグへの展開
田村朋則
生体機能関連化学部会 NEWS LETTER, 32, 3, 8-12 (2017)
5. 生細胞における細胞内局在特異的分子標識法
藤沢有磨、田村朋則、浜地格
生体の科学, 68, 428-429 (2017)
4. タンパク質有機化学の進歩と生細胞への展開
西川雄貴、田村朋則、浜地格
有機合成化学協会誌, 74, 521-531 (2016)
3. Labeling proteins by affinity-guided DMAP chemistry.
Tomonori Tamura, Itaru Hamachi
Methods in Molecular Biology, 1266, 229-242 (2015)
2. Recent progress in design of protein-based fluorescent biosensors and their cellular applications.
Tomonori Tamura, Itaru Hamachi
ACS Chemical Biology, 9, 2708-2717 (2014)
1. 内在性タンパク質に対する選択的ケミカルラベリングの新手法: リガンド指向型トシル化学によるラベル化の可能性
田村朋則、浜地格
化学と生物, 48, 160-162 (2010)