Assistant professor   中村 秀樹 - Hideki Nakamura -

Paper List

19. Tyrosinase-based proximity labeling in living cells and in vivo.
H Zhu*, J H Oh, Y Matsuda, T Mino, M Ishikawa, H Nakamura, M Tsujikawa, H Nonaka, I Hamachi*
Journal of the American Chemical Society, 146,11,7515-7523 (2024). doi: 10.1021/jacs.3c13183

18. Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules.
Nakamura H
Methods in Molecular Biology (2021), 2312: 253-276. doi: 10.1007/978-1-0716-1441-9_15.

17. 生きた細胞内に人工のハイドロゲル凝縮体をつくる合成生物学技術iPOLYMER.
中村 秀樹
生物物理 (2021), 61(3):166-170. doi: 10.2142/biophys.61.166. (Review)

16. Rational design and implementation of a chemically inducible heterotrimerization system.
H D Wu, M Kikuchi, O Dagliyan, A K Aragaki, H Nakamura, N V Dokholyan, T Umehara & T Inoue
Nature Methods (2020), 17:928–936. doi: 10.1038/s41592-020-0913-x.

15. Harnessing biomolecular condensates in living cells.
Nakamura H#, DeRose R, Inoue T#. (#corresponding authors)
Journal of Biochemistry (2019), 166(1):13-27. doi: 10.1093/jb/mvz028. (Review)

14. Stimulation-induced changes in diffusion and structure of calmodulin and calmodulin-dependent protein kinase proteins in neurons.
Heidarinejad M, Nakamura H, Inoue T
Neuroscience Research (2018), 136:13-32. doi: 10.1016/j.neures.2018.01.003.

13. Intracellular production of hydrogels and synthetic RNA granules by multivalent interactions.
Nakamura H*, Lee AA*, Afshar AS, Watanabe S, Rho E, Razavi S, Suarez A, Lin Y-C, Tanigawa M, Huang B, DeRose R, Bobb D, Hong W, Gabelli SB, Goutsias J, Inoue T. (*equally contributed)
Nature Materials (2018), 17(10):79-89, doi: 10.1038/nmat5006.

12. Toward total synthesis of cell function: Reconstituting cell dynamics with synthetic biology.
Kim A.K., DeRose R., Ueno T., Lin B., Komatsu T., Nakamura H., Inoue T.
Sci. Signal. (2016), 9 (414):re1, doi: 10.1126/scisignal.aac4779. (Review)

11. Optical detection of neuron connectivity by random access two-photon microscopy.
Safeghat N., Heidarinejad M., Murata N., Nakamura H., Inoue T.
J. Neurosci. Methods (2016) ,263:48-56, doi:10.1016/j.neumeth.2016.01.023.

10. Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo.
Inoue, T., Narita, K., Nonami, Y., Nakamura, H., Takeda, S.
J. Vis. Exp. (2015), (101): e52991, doi:10.3791/52991.

9. TRPV4 regulates the integrity of the blood-cerebrospinal fluid barrier and modulates transepithelial protein transport.
Narita K., Sasamoto S., Koizumi S., Okazaki S., Nakamura H., Inoue T., Takeda S.
FASEB J. (2015), 29(6):227-2259, doi:10.1096/fj.14-261396.

8. Chemically inducible diffusion trap at cilia reveals molecular sieve-like barrier.
Lin YC, Niewiadomski P*, Lin B*, Nakamura H*, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi R, Inoue T., (*equally contributed)
Nature Chem. Biol. (2013), 9(7):437-443, doi: 10.1038/nchembio.1252.

7. Intracellular click reaction with a fluorescent chemical Ca2+ indicator to prolong its cytosolic retention.
Takei Y, Murata A, Yamagishi K, Arai S, Nakamura H, Inoue T, Takeoka S.
Chem. Commun. (2013), 49(66):7313-7315, doi: 10.1039/c3cc42489h.

6. Developmental changes in ciliary motility on choroid plexus epithelial cells during the perinatal period.
Nonami Y, Narita K, Nakamura H, Inoue T, Takeda S.
Cytoskeleton, (2013), 70(12):797-803, doi: 10.1002/cm.21132.

5. Cationic amino acid-based lipids as effective non-viral gene delivery vectors for primary neurons.
Aoshima Y, Hokama RR, Sou K, Sarker SR, Iida N, Nakamura H, Inoue T, Takeoka S.
ACS Chem. Neurosci., (2013), 4(12):1514-1519, doi: 10.1002/cm.21132.

4. Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals.
Narita K, Kozuka-Hata H, Nonami Y, Ao-Kondo H, Suzuki T, Nakamura H, Yamakawa K, Oyama M, Inoue T, Takeda S.
Biol. Open, (2012), 1(8):815-825, doi: 10.1242/bio.20121081.

3. Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells.
Nakamura H, Bannai H, Inoue T, Michikawa T, Sano M, Mikoshiba K.
J. Biol. Chem. (2012), 287(29):24563-24572, doi: 10.1074/jbc.M111.311399.

2. Highly cooperative dependence of sarco/endoplasmic reticulum calcium ATPase SERCA2a pump activity on cytosolic calcium in living cells.
Satoh K, Matsu-ura T, Enomoto M, Nakamura H, Michikawa T, Mikoshiba K.
J. Biol. Chem. (2011), 286(23):20591-20599, doi: 10.1074/jbc.M110.204685.

1. Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 is regulated by actin filaments and 4.1N in neuronal dendrites.
Fukatsu K, Bannai H, Zhang S, Nakamura H, Inoue T, Mikoshiba K.
J. Biol. Chem. (2004), 279(47):48976-48982

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